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Peripheral Blood CD25+CD4+T Cells in Childhood Patients Treated with Allogeneic Stem Cell Transplantation

Issue: Vol.6, No.2 - April 2007

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Article Type: Manuscript

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  1. Dr Kenichi Sugita
    Department of Pediatrics, Dokkyo Medical University
  2. Tatsuo Tsuboi
  3. Yuya Sato
  4. Hidemitsu Kurosawa
  5. Osamu Arisaka

We here studied regulatory T cells (CD25+CD4+T cells) post-allogeneic stem cell transplantation (SCT) as an important marker in the understanding of immune reconstitution post-SCT. 22 patients with SCT were enrolled. Peripheral blood was obtained and surface antigen was examined by flow cytometry. The percentage and absolute count of CD4+T cells were low at three and six months post-SCT. The percentage of CD25 expression on CD4+T cells at any stage post-SCT was within normal value, whereas the absolute count of CD25+CD4+T cells was low. In contrast, the percentage of HLA-DR expression, an active marker, on CD4+T cells was high at three and six months post-SCT, and the absolute count of HLA-DR+CD4+T cells increased at six and 18 months post-SCT. As a result we found that the difference between CD25 and HLA-DR expression was important as a marker for a development of GVHD. The percentage in subtracting HLA-DR from CD25 expression clearly tends to become positive on periods post-SCT and in fact that in every patient with PSL-therapy for GVHD was negative. We concluded that it took a long period for CD25+CD4+T cell count to normalize and GVHD strongly depended on the difference between CD25 and HLA-DR expression on CD4+T cells.

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